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MW-Deng, SJ-Wei, TL-Yew, PH-Lee, TY-Yang, HY-Chu, SC-Hung*. Cell therapy with G-CSF-mobilized stem cells in a rat osteoarthritis model. Cell Transplantation. 2014
2014 Mar 24. [Epub ahead of print]
Cell therapy with G-CSF-mobilized stem cells in a rat osteoarthritis model.
Abstract
G-CSF-mobilized peripheral blood stem cells (gm-PBSCs) offer a convenient cell source for treatment of hematopoietic and vascular disorders. Whether gm-PBSCs provide beneficial effects on skeleton diseases, such as osteoarthritis (OA), remain unknown. This study was undertaken to address the hypothesis that gm-PBSCs promote articular regeneration in OA. Here, we studied the effect of singledose intra-articular injection of gm-PBSCs from male donors delivered in hyaluronic acid (HA) on papain-induced OA in the knee joints of female Sprague Dawley (SD) rats. Contralateral OA knee joints received single-dose HA alone serve as vehicle controls. We evaluated the histologic changes in glycosaminoglycan, type II collagen, type X collagen, modified Mankin score and cell apoptosis rate in the articular cartilage of rat knees. We demonstrated that gm-PBSCs were mobilized to the peripheral blood via G-CSF infusion for 5 days in SDrats with increasing CD34+ percentage up to 55 folds. We showed that gm-PBSCs inhibit progression of papain-induced OA via reducing articular surface irregularity, fibrillation and erosion, preventing cellular necrosis and loss of chondrogenic proteins, such as glycosaminoglycan and type II collagen, at both 3 and 6 weeks after treatment. Moreover, gm-PBSCs reduced modified Mankin scores and cellular apoptosis rate compared with HA alone. Our findings demonstrate that HA plus gm-PBSCs, rather than HA alone, inhibits progression of OA in rats in vivo. Thus, intra-articular injection of gm-PBSCs is a convenient protocol for treating OA with consistent beneficial effects.
 
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